LOCUS       sequence-153807-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for N-terminal EGFP-AID tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     pBabe Neo OsTIR1-9Myc
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA5-FRT-TO-EGFP-AID
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(172..174,175..177,178..888)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(172..174,175..177,178..888)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(217..238)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(478..497)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     825..846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgccgccac catggtgagc
      181 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      241 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      301 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      361 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      421 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      481 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      541 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      601 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      661 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      721 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      781 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      841 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggc tagcggcgga
      901 tctggcagtg tcgagctgaa tctgag
//
