LOCUS       sequence-153810-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for C-terminal AID-EGFP tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     pBabe Neo OsTIR1-9Myc
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA5-FRT-TO-AID-EGFP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             445..648
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga gctcggatcc
      181 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctccggagcc
      241 gccgctgctg gcggatctgg cagtgtcgag ctgaatctga gggagactga gctgtgtctt
      301 ggtcttcccg gtggagatac agtggctccg gtaaccggaa acaagagagg gttctcagag
      361 acggttgatc tgaagctaaa tctgaataat gagcctgcaa acaaggaagg atctacgact
      421 catgacgtcg tgacttttga ttccaaggag aagagtgctt gtcctaaaga tccagccaaa
      481 cctccggcca aggcacaagt tgtgggatgg ccaccggtga gatcataccg gaagaacgtg
      541 atggtttcct gccaaaaatc aagcggtggc ccggaggcgg cggcgttcgt gaaggtatca
      601 atggacggag caccgtactt gaggaaaatc gatttgagga tgtataaaag ctacgatgag
      661 ctttctaatg ctttgtccaa catgttcagc tcttttacca tgggcaaaca tggaggagaa
      721 gaaggaatga tagacttcat gaatgagagg aaattgatgg atttggtgaa tagctgggac
      781 tatgttccct cttatgaaga caaagacggt gattggatgc tcgtcggcga cgttccttgg
      841 ccaatgttcg tcgatacatg caagcgttta cgtctcatga aaggatcgga tgccattggt
      901 ctcgctccga gg
//
