LOCUS       sequence-153812-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single fluorescent protein biosensor for maltose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pTKEI-Mal-B2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(40..63)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      171..180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgatgg tgatggtgac cgctcttggt gatacgagtc tgcgcgtctt tcagggcttc
       61 atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa
      121 agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt ccatggtggc
      181 ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcta cggcacccag
      241 cggtttgtct ttattaaccg cttccagacc ttcatcagtc agcagatagt tttcgaggaa
      301 ctctttcgcc agctctttgt tcggactggc ggcgttaata cctgcgctca gcacgccaac
      361 gaacggtttg gatggttgac ccttgaaggt cggcagtacc gttacaccat aattcacttt
      421 gctggtgtcg atgttggacc atgcccacgg gccgttgatg gtcatcgctg tttcgccttt
      481 attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat
      541 caggtcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt
      601 aatgtcgtac ttgccgtttt catacttacg actgttgaag ttatattcaa gcttatggcc
      661 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt taaccagagt
      721 gtcgccctcg aacttaacct cagcgcgtgt cttatagttg ccgtcgtcct tgaagaagat
      781 ggtgcgctcc tgaatgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      841 gtggtcggg
//
