LOCUS       sequence-153818-        6581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F
  TITLE     Physical and functional interactions between the human DNMT3L 
            protein and members of the de novo methyltransferase family.
  JOURNAL   J Cell Biochem. 2005 Aug 1;95(5):902-17.
  PUBMED    15861382
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2005-08-1"; volume: "95(5)"; pages: "902-17"
COMMENT     Sequence Label: pcDNA3/Myc-DNMT3L
FEATURES             Location/Qualifiers
     source          1..6581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2033..2052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2604..2807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2758..2778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2852..2871
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2852..2870
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2884..2893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2893..2922
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4123..4141)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4124..4141)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4161..4178)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4167..4391
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4437..4865
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4524..4543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4734..4755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4874..4894)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4879..5208
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5059..5194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5121..5140
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5275..6069
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5329..5348)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5939..5958
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6243..6364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6280..6299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6334..6353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6413..6429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6413..6429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6426..6448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6437..6453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6461..6467,6468..6485,6486..6491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6506..6527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     2041 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     2101 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     2161 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     2821 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     2881 cttgccacca tggagcagaa gctgatctca gaggaggacc tggaattcat ggcggccatc
     2941 ccagccctgg acccagaggc cgagcccagc atggacgtga ttttggtggg atccagtgag
     3001 ctctcaagct ccgtttcacc cgggacaggc agagatctta ttgcatatga agtcaaggct
     3061 aaccagcgaa atatagaaga catctgcatc tgctgcggaa gtctccaggt tcacacacag
     3121 caccctctgt ttgagggagg gatctgcgcc ccatgtaagg acaagttcct ggatgccctc
     3181 ttcctgtacg acgatgacgg gtaccaatcc tactgctcca tctgctgctc cggagagacg
     3241 ctgctcatct gcggaaaccc tgattgcacc cgatgctact gcttcgagtg tgtggatagc
     3301 ctggtcggcc ccgggacctc ggggaaggtg cacgccatga gcaactgggt gtgctacctg
     3361 tgcctgccgt cctcccgaag cgggctgctg cagcgtcgga ggaagtggcg cagccagctc
     3421 aaggccttct acgaccgaga gtcggagaat ccccttgaga tgttcgaaac cgtgcctgtg
     3481 tggaggagac agccagtccg ggtgctgtcc ctttttgaag acatcaagaa agagctgacg
     3541 agtttgggct ttttggaaag tggttctgac ccgggacaac tgaagcatgt ggttgatgtc
     3601 acagacacag tgaggaagga tgtggaggag tggggaccct tcgatcttgt gtacggcgcc
     3661 acacctcccc tgggccacac ctgtgaccgt cctcccagct ggtacctgtt ccagttccac
     3721 cggctcctgc agtacgcacg gcccaagcca ggcagcccca ggcccttctt ctggatgttc
     3781 gtggacaatc tggtgctgaa caaggaagac ctggacgtcg catctcgctt cctggagatg
     3841 gagccagtca ccatcccaga tgtccacggc ggatccttgc agaatgctgt ccgcgtgtgg
     3901 agcaacatcc cagccataag gagcaggcac tgggctctgg tttcggaaga agaattgtcc
     3961 ctgctggccc agaacaagca gagctcgaag ctcgcggcca agtggcccac caagctggtg
     4021 aagaactgct ttctccccct aagagaatat ttcaagtatt tttcaacaga actcacttcc
     4081 tctttataag tcgacctcga gcatgcatct agagggccct attctatagt gtcacctaaa
     4141 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4201 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4261 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4321 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4381 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4441 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4501 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4561 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4621 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4681 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4741 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4801 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4861 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4921 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4981 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5041 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5101 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5161 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5221 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5281 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5341 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5401 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5461 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5521 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5581 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5641 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5701 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5761 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5821 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5881 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5941 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6001 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6061 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6121 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6181 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6241 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6361 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct g
//
