LOCUS       sequence-153819-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast two-hybrid expression vector, expresses Gal4BD fused to 
            Arabidopsis CRY2 (residues 1-535).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
  TITLE     Optimized second-generation CRY2-CIB dimerizers and photoactivatable
            Cre recombinase.
  JOURNAL   Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
  PUBMED    27065233
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063."
COMMENT     Sequence Label: Gal4BD-CRY2(535)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..609
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 aaaagtttgc tgtcttgcta tcaagtataa atagacctgc aattattaat cttttgtttc
       61 ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct
      121 ataccaagca tacaatcaac tccaagcttg aagcaagcct cctgaaagat gaagctactg
      181 tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa
      241 aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc
      301 aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg
      361 gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat
      421 tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa
      481 gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag
      541 catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg
      601 actgtatcgc cggaattcat gaagatggac aaaaagacta tagtttggtt tagaagagac
      661 ctaaggattg aggataatcc tgcattagca gcagctgctc acgaaggatc tgtttttcct
      721 gtcttcattt ggtgtcctga agaagaagga cagttttatc ctggaagagc ttcaagatgg
      781 tggatgaaac aatcacttgc tcacttatct caatccttga aggctcttgg atctgacctc
      841 actttaatca aaacccacaa cacgatttca gcgatcttgg attgtatccg cgttaccggt
      901 gctacaaaag tcgtctttaa ccacctctat gatcctgttt cgttagttcg ggaccatacc
      961 gtaaaggaga agctggtgga acgtgggatc tctgtgca
//
