LOCUS       sequence-153821-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, 
            Singer RH
  TITLE     In vivo dynamics of RNA polymerase II transcription.
  JOURNAL   Nat Struct Mol Biol. 2007 Sep;14(9):796-806. Epub 2007 Aug 5.
  PUBMED    17676063
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2007-09"; volume: "14(9)"; pages: "796-806. Epub 
            2007 Aug 5"
COMMENT     Sequence Label: pYFP-RPB1aAmr
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3..68
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      620..629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
       61 cgggatccac cggatctagc atgcacgggg gtggcccccc ctcgggggac agcgcatgcc
      121 cgctgcgcac catcaagaga gtccagttcg gagtcctgag tccggatgaa ctgaagcgaa
      181 tgtctgtgac ggagggtggc atcaaatacc cagagacgac tgagggaggc cgccccaagc
      241 ttggggggct gatggacccg aggcaggggg tgattgagcg gactggccgc tgccaaacat
      301 gtgcaggaaa catgacagag tgtcctggcc actttggcca cattgaactg gccaagcctg
      361 tgtttcacgt gggcttcctg gtgaagacaa tgaaagtttt gcgctgtgtc tgcttcttct
      421 gctccaaact gcttgtggac tctaacaacc caaagatcaa ggatatcctg gctaagtcca
      481 agggacagcc caagaagcgg ctcacacatg tctacgacct ttgcaagggc aaaaacatat
      541 gcgagggtgg ggaggagatg gacaacaagt tcggtgtgga acaacctgag ggtgacgagg
      601 atctgaccaa agaaaagggc catggtggct gtgggcggta ccagcccagg atccggcgtt
      661 ctggcctaga gctgtatgcg gaatggaagc acgttaatga ggactctcag gagaagaaga
      721 tcctgctgag tccagagcga gtgcatgaga tcttcaaacg catctcagat gaggagtgtt
      781 ttgtgctggg catggagccc cgctatgcac ggccagagtg gatgattgtc acagtgctgc
      841 ctgtgccccc
//
