LOCUS       sequence-153826-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CRYAA_pCSdest
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(659..683)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(751..770)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgga tgtgaccatc cagcacccct ggttcaagcg caccctgggg
      181 cccttctacc ccagccggct gttcgaccag tttttcggcg agggcctttt tgagtatgac
      241 ctgctgccct tcctgtcgtc caccatcagc ccctactacc gccagtccct cttccgcacc
      301 gtgctggact ccggcatctc tgaggttcga tccgaccggg acaagttcgt catcttcctc
      361 gatgtgaagc acttctcccc ggaggacctc accgtgaagg tgcaggacga ctttgtggag
      421 atccacggaa agcacaacga gcgccaggac gaccacggct acatttcccg tgagttccac
      481 cgccgctacc gcctgccgtc caacgtggac cagtcggccc tctcttgctc cctgtctgcc
      541 gatggcatgc tgaccttctg tggccccaag atccagactg gcctggatgc cacccacgcc
      601 gagcgagcca tccccgtgtc gcgggaggag aagcccacct cggctccctc gtcctaaaac
      661 ccagctttct tgtacaaagt ggtgatcctc tcgagcctct agaactatag tgagtcgtat
      721 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      781 cagtgaaaa
//
