LOCUS       sequence-153827-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CRYAA_pCSdest
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(588..612)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(666..684)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(667..684)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gttttaggac gagggagccg aggtgggctt ctcctcccgc gacacgggga tggctcgctc
      121 ggcgtgggtg gcatccaggc cagtctggat cttggggcca cagaaggtca gcatgccatc
      181 ggcagacagg gagcaagaga gggccgactg gtccacgttg gacggcaggc ggtagcggcg
      241 gtggaactca cgggaaatgt agccgtggtc gtcctggcgc tcgttgtgct ttccgtggat
      301 ctccacaaag tcgtcctgca ccttcacggt gaggtcctcc ggggagaagt gcttcacatc
      361 gaggaagatg acgaacttgt cccggtcgga tcgaacctca gagatgccgg agtccagcac
      421 ggtgcggaag agggactggc ggtagtaggg gctgatggtg gacgacagga agggcagcag
      481 gtcatactca aaaaggccct cgccgaaaaa ctggtcgaac agccggctgg ggtagaaggg
      541 ccccagggtg cgcttgaacc aggggtgctg gatggtcaca tccatggtgc ctgctttttt
      601 gtacaaactt gtgatccttg aattcgaatc gatgggatcc tgcaaaaaga acaagtagct
      661 tgtattctat agtgtcacct aaatcaagct tgctccgacg tccc
//
