LOCUS       sequence-153828-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DNMT3L_pCSdest
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggc ggccatccca gccctggacc cagaggccga gcccagcatg
      181 gacgtgattt tggtgggatc cagtgagctc tcaagctccg tttcacccgg gacaggcaga
      241 gatcttattg catatgaagt caaggctaac cagcgaaata tagaagacat ctgcatctgc
      301 tgcggaagtc tccaggttca cacacagcac cctctgtttg agggagggat ctgcgcccca
      361 tgtaaggaca agttcctgga tgccctcttc ctgtacgacg atgacgggta ccaatcctac
      421 tgctccatct gctgctccgg agagacgctg ctcatctgcg gaaaccctga ttgcacccga
      481 tgctactgct tcgagtgtgt ggatagcctg gtcggccccg ggacctcggg gaaggtgcac
      541 gccatgagca actgggtgtg ctacctgtgc ctgccgtcct cccgaagcgg gctgctgcag
      601 cgtcggagga agtggcgcag ccagctcaag gccttctacg accgagagtc ggagaatccc
      661 cttgagatgt tcgaaaccgt gcctgtgtgg aggagacagc cagtccgggt gctgtccctt
      721 tttgaagaca tcaagaaaga gctgacgagt ttgggctttt tggaaagtgg ttctgacccg
      781 ggacaactga agcatgtggt tgatgtcaca gacacagtga ggaaggatgt ggaggagtgg
//
