LOCUS       sequence-153830-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PCBP3_pCSdest
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgga gtccaaggtc tcagaaggtg gcctgaatgt gaccctcacc
      181 atccgcctgc tgatgcatgg aaaggaagtt ggaagcatca tcgggaagaa aggagaaact
      241 gtgaagaaga tgcgtgagga gagtggtgca aggatcaaca tctcagaggg aaactgccca
      301 gagaggattg tgaccatcac aggccccaca gacgccatct tcaaggcctt tgccatgatc
      361 gcatacaagt ttgaggagga tatcatcaac tccatgagca acagccctgc caccagcaag
      421 cccccagtga cgctgaggct ggtggtgcct gccagccagt gcgggtccct gatcggcaaa
      481 ggaggctcca agatcaagga gatcagggag tccacaggtg cccaggtgca ggtggctggg
      541 gacatgctgc ccaactccac ggagcgagcg gtgaccatct cggggacccc agatgccatc
      601 atccagtgcg tcaagcagat ctgtgtggtc atgctggagg cctacacaat ccagggacag
      661 tatgccatcc ctcacccgga tttgaccaag ctccaccagt tggccatgca gcaaaccccc
      721 tttcctcccc tcggacagac caaccccgct ttccccggag aaa
//
