LOCUS       sequence-153838-        9987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression plasmid for MycLAP-STIL 5A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO MycLAP-STIL 5A
FEATURES             Location/Qualifiers
     source          1..9987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1154..1183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1187..1189,1190..1900)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1187..1189,1190..1900)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1229..1250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1490..1509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1837..1858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1946..1966
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1979..2023
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(5939..5956)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5945..6169
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6302..6321)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6453..6500
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6508..7530
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6508..7530
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7660..7781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7697..7716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7751..7770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7830..7846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7830..7846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7843..7865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7854..7870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7878..7884,7885..7902,7903..7908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7923..7944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8061..8078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8232..8820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8312..8331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8991..9782,9783..9851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9614..9633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9852..9956)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccc cggggaattc ggcagatccc gaggtccgac
     1021 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
     1081 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
     1141 tgccccgcgg atggagcaga agctcatctc agaagaagac ctcgacgtga gcaagggcga
     1201 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1261 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1321 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1381 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1441 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1501 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1561 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1621 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1681 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1741 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1801 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1861 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggagggg atcggtggag
     1921 ctccaccggt ggcggacgct ctagagagaa tctttatttt cagggcgccg ccaaattcaa
     1981 agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggaggtg gatccactag
     2041 tctcgagcct atatatcctt ttgcacggcc ccagatgaat accaggtttc cttcaagcag
     2101 gatggtacct ttccactttc ctccatcaaa atgtgcactt tggaacccaa cgccaactgg
     2161 agatttcatc tacttacatc tcagttacta cagaaatcca aagcttgtgg tgactgagaa
     2221 gaccatccga cttgcttatc gtcatgctaa gcagaataaa aaaaattcgt catgcttttt
     2281 acttggttct ctgacagcag acgaagatga agaaggtgta acattgacag tagatcgctt
     2341 tgatcctggt cgagaagtac ctgaatgcct agaaataacc cctactgctt ctcttcctgg
     2401 ggactttttg attccatgca aagttcatac tcaagaactt tgttcaagag aaatgatagt
     2461 tcacagtgta gatgacttca gttcagcttt aaaggctcta cagtgccata tatgtagcaa
     2521 agattccttg gactgtggta agctgctttc cctaagagtt catatcactt ccagggagag
     2581 tttggacagt gtggaatttg acttgcattg ggcagcagta actctagcaa ataactttaa
     2641 atgcacacct gtgaagccca tccccattat tccaacagct ctggcaagaa acttgagcag
     2701 taatctgaat atttctcaag ttcaagggac ttataaatat ggatatctta ccatggatga
     2761 aacacgcaaa ttgttacttt tgttggaatc tgatcccaag gtttattctc taccattggt
     2821 gggaatttgg ctgtctggaa ttacacatat ctatagtcct caggtatggg cttgctgttt
     2881 gcgatacata ttcaattctt ctgttcaaga aagggttttt tcagaatctg gaaatttcat
     2941 catagttctc tattctatga cacataagga acctgagttt tatgaatgct tcccttgtga
     3001 tggcaagata cctgactttc ggtttcagtt gctaaccagt aaggaaacat tacatctttt
     3061 caaaaatgtt gaacctcctg acaaaaatcc aatccgttgt gaactgagcg ctgaaagcca
     3121 aaatgcagaa acagagtttt tcagtaaggc ttccaagaat ttttcaatta agaggtcttc
     3181 ccaaaagtta tcttctggga agatgccaat acatgatcac gactctggtg ttgaagatga
     3241 agatttttct ccaagaccaa ttcctagtcc tcatccagtg agtcagaaga tttctaagat
     3301 ccaaccatca gttcctgaac tttcacttgt gttggatggc aatttcatag aatcaaaccc
     3361 tctgcctact ccattggaaa tggtgaataa tgaaaatcct cctttgatta accacttgga
     3421 acacttgaag ccattgcaac cccagcttta tgatgagaaa cacagtccag aagttgaagc
     3481 tggagagcct tccttgagag gaataccaaa tcagttaaac caggataaac cagctctttt
     3541 gagacactgc aaagtaagac agccacctgc ctataagaaa gggaaccccc ataccaggaa
     3601 cagtattaaa ccatcttctc ataatgggcc atctcatgat atatttgaaa aactccaaac
     3661 cgtgtctgcc ggaaatgtac aaaacgaaga gtatcctata agaccctcca cacttaattc
     3721 taggcagtct tctcttgccc cgcagtccca accacacgat tttgtttttt caccccataa
     3781 ttcaggaaga ccaatggaac ttcagatacc tactccccca ctgccatctt actgttccac
     3841 aaacgtttgc aggtgttgtc agcatcatag tcatattcaa tatagtccgc taaattcttg
     3901 gcaaggagca aacacagttg gctccattca agatgtccag tctgaagccc ttcaaaagca
     3961 ttcattattt cacccaagtg gatgtccagc cctgtactgt aatgcattct gttcttcaag
     4021 tagtcctata gccttgagac ctcagggaga tatgggcagt tgttctcccc acagcaatat
     4081 tgaaccatcg cctgtggcaa gaccgccttc acatatggac ttatgtaacc cacagccttg
     4141 cacagtgtgc atgcacacac ccaagactga gtcagataat ggaatgatgg gactatctcc
     4201 agatgcatat cggttcctca cagaacaaga cagacagcta agactacttc aggcacagat
     4261 tcagcgtttg ttggaagcac agtctctgat gccctgttcc cctaagacaa ctgctgttga
     4321 agacacagtg caagctggaa gacaaatgga gttggtttct gtggaagcac agtcttcccc
     4381 tggcttgcac atgagaaaag gtgtaagcat tgctgtgagc acaggtgcta gcttgttttg
     4441 gaatgcagca ggtgaggatc aagagcctga ctctcaaatg aagcaagatg ataccaaaat
     4501 ttccagtgag gacatgaatt tttctgtcga tattaataat gaagtcacaa gtcttccagg
     4561 tagtgcatct tcattaaaag cagttgatat tcccagtttt gaagagagca acattgctgt
     4621 ggaagaagaa tttaaccagc cactttctgt atccaactct tctctagttg tgagaaaaga
     4681 acctgatgta cctgtgttct ttccaagtgg ccagctggca gaaagtgtaa gcatgtgttt
     4741 acagactgga ccaacagggg gtgccagtaa caattctgaa acatcagagg aaccaaaaat
     4801 tgagcatgta atgcaaccct tgcttcatca accatcagat aaccagaaaa tttaccagga
     4861 tttattgggt caagtaaacc acctattaaa tagttcctcc aaggaaactg agcagccgtc
     4921 taccaaagca gtaattatca gtcatgaatg caccagaacc caaaacgttt accatacaaa
     4981 gaaaaaaaca catcattcaa gactggtgga caaagattgt gtccttaatg caactcttaa
     5041 gcaactaaga agccttggag taaaaattga ttctcccact aaagtgaaga aaaatgcaca
     5101 taacgtggat cacgccagtg tgttggcatg catcagccca gaagcagtga tctctggatt
     5161 aaactgcatg tcatttgcta atgttggcat gagcggctta agccccaatg gtgtggattt
     5221 gagcatggag gcaaatgcta tagctctgaa atatttaaat gaaaatcagc tgtcacaact
     5281 gtctgtcact cgatcgaacc aaaataattg tgacccattc agccttctcc atattaatac
     5341 agacagagcc acagtggggc ttgctttaat tgcaccaaac aacatggcat ttgcagccaa
     5401 aaaatatatg aagagatatg gactcctaca aagcagtgac aatagtgaag atgaagagga
     5461 acctcccgac aatgcagata gcaagagtga atatttattg aatcagaacc ttaggtccat
     5521 acccgaacag cttggtggtc agaaagagcc ttctaagaat gaccatgaaa taattaattg
     5581 ttctaactgt gaatctgtgg ggaccaacgc agatacgcca gtattgagaa atattacaaa
     5641 tgaagttttg cagacaaaag caaaacagca gttgactgaa aagccagctt tcttagtaaa
     5701 gaaccttaaa ccaagtcctg cagtgaacct tcgaaccggg aaagcagagt tcactcaaca
     5761 tcctgagaaa gaaaatgaag gggacattac aatttttcct gaaagtttgc aaccttctga
     5821 aacgctaaag cagatgaata gcatgaattc agtaggcacc ttcttagatg taaaacgtct
     5881 cagacagtta ccaaaattat tttaagcggc cgcgggcccg tttaaacccg ctgatcagcc
     5941 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6001 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6061 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6121 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6181 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6421 aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct
     6481 ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag
     6541 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     6601 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     6661 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     6721 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     6781 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     6841 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     6901 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     6961 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     7021 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     7081 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     7141 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     7201 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     7261 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     7321 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     7381 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     7441 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     7501 cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc
     7561 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7621 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7801 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9961 tttccccgaa aagtgccacc tgacgtc
//
