LOCUS       sequence-153839-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for MycLAP-STIL 5A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO MycLAP-STIL 5A
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             242..271
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(275..277,278..790)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(317..338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(578..597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgttta
       61 aacttaagct tggtaccccg gggaattcgg cagatcccga ggtccgacag cgcccggccc
      121 agatccccac gcctgccagg agcaagccga gagccagccg gccggcgcac tccgactccg
      181 agcagtctct gtccttcgac ccgagccccg cgccctttcc gggacccctg ccccgcggat
      241 ggagcagaag ctcatctcag aagaagacct cgacgtgagc aagggcgagg agctgttcac
      301 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      361 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      421 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      481 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      541 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      601 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      661 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      721 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      781 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      841 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
      901 agaccccaac gagaagcgcg atcacatggt cc
//
