LOCUS       sequence-153840-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for MycLAP-STIL coiled-coil deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO MycLAP-STIL ∆CC
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             238..267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(271..273,274..786)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(313..334)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(574..593)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     921..942
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttaaact
       61 taagcttggt accccgggga attcggcaga tcccgaggtc cgacagcgcc cggcccagat
      121 ccccacgcct gccaggagca agccgagagc cagccggccg gcgcactccg actccgagca
      181 gtctctgtcc ttcgacccga gccccgcgcc ctttccggga cccctgcccc gcggatggag
      241 cagaagctca tctcagaaga agacctcgac gtgagcaagg gcgaggagct gttcaccggg
      301 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      361 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      421 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      481 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      541 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      601 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      661 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      721 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      781 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      841 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
      901 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgg
//
