LOCUS       sequence-153847-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for Plk4 with multi-phospho degron 
            deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO Plk4 ∆24-mCherry
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(282..299)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    288..512
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(645..664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    796..843
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagtaaagc ggccgcgggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
      301 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      361 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      421 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      481 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
      541 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      601 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      781 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
      841 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaa
//
