LOCUS       sequence-153850-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for kinase dead Plk4 with multi-phospho
            degron deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..715)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(283..302)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     552..570
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgctttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
       61 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
      121 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
      181 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
      241 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
      301 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      361 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
      421 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
      481 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca
      541 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
      601 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
      661 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accattggat
      721 ccgccccggg atgaaaatta ggagtcggat tagaaaacat caaaaggatg gaagacagac
      781 actgtaattt ctgtttgatg tagtctggta atttttcatt ttctccatac ctagttgttt
      841 gaccatttgg tgaggtataa ctgatagaag acactcctgc ctgcacaa
//
