LOCUS       sequence-153852-       10481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Please see Addgene item #11367 for an updated version of this 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, 
            Erdjument-Bromage H, Tempst P, Sabatini DM
  TITLE     Rictor, a novel binding partner of mTOR, defines a 
            rapamycin-insensitive and raptor-independent pathway that regulates 
            the cytoskeleton.
  JOURNAL   Curr Biol 2004 Jul 27;14(14):1296-302.
  PUBMED    15268862
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol";
            date: "2004-07-27"; volume: "14(14)"; pages: "1296-302"
COMMENT     Sequence Label: myc-Rictor
FEATURES             Location/Qualifiers
     source          1..10481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        198..577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        578..781
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     732..752
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     778..802
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1012..1030
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1012..1029
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1043..1062
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1147..1156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1156..1185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      6965..6974
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6978..7112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7015..7034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7069..7088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7181..7538
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7181..7372
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7204..7224)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7389..7524
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7558..7575)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7558..7574)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7567..7589)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7787..8242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7874..7893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8084..8105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8259..8278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8378..8400
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8438..8456)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8524..8628
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8629..8697,8698..9489)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8847..8866)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9660..10248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10149..10168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10402..10419
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      181 ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat caattacggg
      241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      781 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      841 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
      901 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
      961 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     1021 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     1081 ctgcacctcg gttctatcga ttgaattccc gaaccgacag tcggtctctt caccaaggcc
     1141 attcgcgcca ccatggagca aaagctcatt tctgaggaag atctcaatgg tggtggtggt
     1201 gggtcgacaa tggcggcgat cggccgcggc cgctctctga agaacctccg agtacgaggg
     1261 cggaatgaca gcggcgagga gaacgtcccg ctggatctga cccgagaacc tcctgataac
     1321 ttaagagaga ttctccaaaa tgtggccaga ttgcagggag tatcaaatat gagaaagcta
     1381 ggccatctga ataactttac taagcttctt tgtgatattg gccacagtga agaaaaactg
     1441 ggctttcact atgaggatat cataatttgt ttgcggttag ctttattaaa tgaagcaaaa
     1501 gaagtgcgag cagcagggct acgagcgctt cgatatctca tccaagactc cagtactctc
     1561 cagaaggtgc taaaattgaa agtggactat ttaatagcta ggtgcattga catacaacag
     1621 agcaacgagg tagagaggac acaagcactt cgattagtca gaaagatgat tactgtgaat
     1681 gcttccttgt ttcctagttc tgtgaccaac tcattaattg cagttggaaa tgatggactt
     1741 caagaaagag acagaatggt ccgagcatgc attgccatta tctgtgaact agcacttcag
     1801 aatccagagg tggtggccct tcgaggtgga ctaaacacca tcttgaaaaa tgtgatcgat
     1861 tgccaattaa gtcgaataaa tgaggcccta attactacaa ttttgcacct tcttaatcat
     1921 ccaaagactc gacagtatgt gcgagctgat gtagaattag agagaatttt agcaccctat
     1981 actgattttc actacagaca tagtccagat acagctgaag gacagctcaa agaagacaga
     2041 gaagcacgat ttctagccag taaaatggga atcatagcaa cattccgatc atgggcaggt
     2101 attattaatt tatgtaaacc tggaaattct gggatccagt ctctaatagg agtactttgc
     2161 ataccaaata tggaaataag gcgaggtcta cttgaagtgc tttatgatat atttcgtctt
     2221 cctctacctg ttgtgactga ggagttcata gaagcactac tcagtgtaga tccagggagg
     2281 ttccaagaca gttggaggct ttcagatggc tttgtggcag ctgaggcaaa aactattctt
     2341 cctcatcgtg ccagatccag gccagacctc atggataatt atttggcact gatactctct
     2401 gcatttattc gtaatggact tttagagggt ctagttgaag tgataacaaa cagtgatgat
     2461 catatctcag ttagagctac catcctttta ggagagcttt tacatatggc aaacacaatt
     2521 cttcctcatt cacatagcca tcatttacac tgcttgccaa ccctaatgaa tatggctgca
     2581 tcctttgata tccccaagga aaagagactg cgagccagtg cagccttgaa ctgtttaaaa
     2641 cgcttccatg aaatgaagaa acgaggacct aagccttata gtcttcattt agaccacatt
     2701 attcagaaag caattgcaac acaccagaaa cgggatcagt atctccgagt tcagaaagat
     2761 atatttatcc ttaaggatac agaggaagct cttttaatta accttagaga tagccaagtc
     2821 cttcaacata aagagaatct tgaatggaat tggaatctta tagggaccat tcttaagtgg
     2881 ccaaatgtaa atctaagaaa ctataaagat gaacagttac acaggtttgt acgaagacta
     2941 ctttattttt acaagcccag cagtaaatta tatgccaacc tggatctgga ttttgccaag
     3001 gccaaacagc tcacggttgt aggttgccag tttacagaat ttcttcttga atctgaagag
     3061 gatgggcaag gctacttaga agatctagta aaggatattg ttcagtggct caatgcttca
     3121 tctggaatga aacccgaaag aagtcttcaa aataatggtt tattgaccac ccttagtcaa
     3181 cactactttt tatttattgg aacactttct tgccaccctc atggagttaa aatgctggaa
     3241 aaatgcagtg tatttcagtg tctccttaat ctttgctcct tgaaaaacca agatcacttg
     3301 ctaaaactta ctgtttctag cttggactat agcagagatg gattggctag agtcatcctt
     3361 tccaaaattt taactgcagc tactgatgcc tgcagactct atgcaacaaa acatttaagg
     3421 gtattattga gagctaatgt tgaattcttt aataattggg gaattgagtt gttagtgacc
     3481 cagctacatg ataaaaacaa aacgatttcc tctgaagctc ttgatatcct cgatgaagca
     3541 tgtgaagaca aggccaatct tcatgctctc attcagatga aaccagcgtt atcccacctt
     3601 ggagacaagg gtttgcttct cctgctgaga tttctctcca ttccaaaagg attttcctat
     3661 ctgaatgaaa gaggttatgt agcaaaacaa ttggaaaagt ggcacaggga atacaactcc
     3721 aaatatgttg acttgattga ggaacaactc aatgaagcac ttactactta ccggaagcct
     3781 gttgatggtg ataactatgt tcgtcggagt aaccaaagat tacagcgtcc tcacgtctac
     3841 ctgcctatac acctttatgg acaactagta caccataaaa caggctgcca tttgttggaa
     3901 gtacagaata ttattacaga actctgtcgt aatgttcgta caccagattt ggataagtgg
     3961 gaagaaatta aaaaactgaa agcatctctt tgggccttgg gaaatatcgg ctcatcaaat
     4021 tggggtctca atttgctaca ggaagaaaac gtgattccag atatactaaa acttgcaaaa
     4081 cagtgtgaag ttctttccat cagagggacc tgtgtatatg tacttgggct catagctaaa
     4141 accaaacaag gctgtgatat tctaaaatgt cacaactggg atgctgtgag gcatagtcgc
     4201 aaacatctgt ggccagtggt tccagatgat gtggaacaac tctgtaatga actttcatct
     4261 atcccaagca ctctaagttt gaactcggag tcaaccagct ctagacataa tagtgaaagt
     4321 gaatctgtgc catcgagtat gttcatattg gaggatgacc ggtttggcag cagctctact
     4381 agtacatttt tccttgatat caatgaagat acagagccaa cattttatga ccgatctgga
     4441 cccataaagg ataaaaattc attccctttc tttgcttcta gtaaacttgt gaagaatcgt
     4501 atcttaaatt cgcttacttt gcctaacaaa aaacatcgta gtagcagtga tccaaaagga
     4561 gggaaattat catctgaaag taagacaagc aacaggcgaa tcagaacact tacggagccc
     4621 agtgttgatt ttaatcatag tgatgatttt acacccatat ccactgtaca gaaaacatta
     4681 caattagaga cttcatttat ggggaataag cacattgaag acactggtag tacaccaagc
     4741 attggagaaa atgacttaaa attcaccaag aattttggta cagagaatca cagagaaaat
     4801 acaagccgag agaggttagt agtagaaagt tcaacgagct cacatatgaa gatacgtagc
     4861 caaagtttca atacagacac tacaacaagt ggcataagtt caatgagctc aagtccttca
     4921 cgagagacag taggtgtaga tgctacaact atggacacag actgtggaag catgagtact
     4981 gtggtaagta ctaaaactat taagacaagc cactatttga cgccacagtc taaccatctg
     5041 tctctctcca aatcaaattc ggtgtccctg gtgcctccag gttcttctca tacgcttcct
     5101 agaagagcac agtcccttaa agcaccctct attgctacaa ttaaaagtct agcagattgt
     5161 aactttagtt acacaagttc tagagatgct tttggctatg ctacactgaa aagactacag
     5221 caacaaagaa tgcatccatc cttatctcac tctgaagctt tggcatctcc agcaaaagat
     5281 gtgctattta ctgataccat caccatgaag gccaacagtt ttgagtccag attaacacca
     5341 agcaggttca tgaaagcctt aagttatgca tcattagata aagaagattt attgagtcct
     5401 attaatcaaa ataccctgca acgatcttcc tcagtgcggt ccatggtgtc cagtgccaca
     5461 tatgggggtt cagatgatta cattggtctt gctctcccgg tggatataaa tgatatattc
     5521 caggtaaagg atattcccta ttttcagaca aaaaacatac caccacatga tgatcgaggt
     5581 gcaagagcat ttgcccatga tgcaggaggt cttccatctg gaactggagg tcttgtaaaa
     5641 aattcttttc acttgctacg acagcagatg agtcttacgg aaataatgaa ttcaatccat
     5701 tcagatgcct ctctgttttt agaaagtaca gaagacactg gactacagga acatacagat
     5761 gataactgcc tttattgtgt ctgtattgaa attctgggtt tccagcccag caaccaactg
     5821 agtgcaatat gtagtcattc agactttcaa gatattccat attctgattg gtgtgagcag
     5881 actatccata atcctttaga agtggttccc tctaagtttt cggggatttc tggatgcagt
     5941 gatggggtgt ctcaagaagg ctcagctagc agcaccaaaa gcacagaatt gttactaggt
     6001 gttaaaacaa ttccagatga tacaccaatg tgccgtatac tccttcgcaa agaagttcta
     6061 agattagtca ttaatttgag tagttcagtt tcaactaaat gtcatgagac tgggctttta
     6121 acaattaagg agaagtatcc tcaaacattt gatgacatat gcctttactc tgaggtttcc
     6181 catttgctgt cacactgcac attcagactt ccgtgtcgga ggttcataca agaattattt
     6241 caagatgtac agtttctaca aatgcatgaa gaagcagagg ctgtgttggc aacaccacca
     6301 aagcaaccta tagttgatac atctgctgaa tcctgacctc atatttatga tggatataga
     6361 tacatactat atatattcat atttgtggat ttcctaaaag cctcagaaaa tacgactgac
     6421 taggcagcaa agacaggagt atcttctgta cactgttccg cagttactgg tacatgaaca
     6481 gttggaactg ctgactttcc taaccaaaac aacttccttc tctcctttgt tgagcctttt
     6541 gaggggttca tgattcatta ccacagtttt aagagtttca gttaccattg tatgcaagag
     6601 ccaagcactg aatacctaca taggttttct attttctttc attttaaaag cgtaatgaca
     6661 gtggaacaat aatgggatat gcagaagcac ccttcacaag ttatttctga atgattttta
     6721 gggtaaataa tacagatgcc ttgtttgtta actaacttgt ggaaagcagg aatcagtgtc
     6781 tctaaggctg catcctatta ccacaatggg gtgtgctata actgctggta ttagagaggg
     6841 aactttggcc ctttcacgtt tttcttaatg tttgtaacac tacttcagag gtttataacc
     6901 tcaaagcaga agaagagcct caacaacccg ggcggccgct aagtaagtaa ggatccccag
     6961 cttggccgcc atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7021 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7081 aactcatcaa tgtatcttat catgtctgga tcgggaatta attcggcgca gcaccatggc
     7141 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     7201 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7261 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7321 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7381 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7441 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7501 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca gcttggcact
     7561 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7621 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7681 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     7741 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     7801 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7861 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7921 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7981 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     8041 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8101 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     8161 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8221 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     8281 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8341 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8401 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     8461 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8521 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8581 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8641 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8701 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8761 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8821 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8881 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8941 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9001 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9061 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9121 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9181 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9241 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9301 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9361 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9421 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9481 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9541 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9601 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10321 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10381 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10441 cgcaaaccgc ctctccccgc gcgttggccg attcattaat g
//
