LOCUS       sequence-153870-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA shuttle vector; module 8E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE15
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        129..204
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(262..279)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(262..278)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(271..293)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(487..506)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     606..628
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(666..684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        752..856
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1075..1094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 gaagacttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      181 gaaaaagtgg caccgagtcg gtgctttttt ttgcttagag accttatcta gatgcattcg
      241 cgaggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      301 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      361 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      421 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      481 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      541 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      601 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      661 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1081 tggcgcggta ttatcccgta ttgacgccgg gca
//
