LOCUS       sequence-153873-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE2
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..653
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      673..925
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    968..992
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta
       61 gagtctcata ttcactctca actcgatcga ggggatctac catgagccca gaacgacgcc
      121 cggccgacat ccgccgtgcc accgaggcgg acatgccggc ggtctgcacc atcgtcaacc
      181 actacatcga gacaagcacg gtcaacttcc gtaccgagcc gcaggaaccg caggagtgga
      241 cggacgacct cgtccgtctg cgggagcgct atccctggct cgtcgccgag gtggacggcg
      301 aggtcgccgg catcgcctac gcgggtccct ggaaggcacg caacgcctac gactggacgg
      361 ccgagtcgac cgtgtacgtc tccccccgcc accagcggac gggactgggc tccacgctct
      421 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
      481 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
      541 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
      601 acttcagcct gccggtgccg ccccgtccgg tcctgcccgt caccgaaatc tgatgacccc
      661 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
      721 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
      781 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
      841 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
      901 gcggtgtcat ctatgttact agatcggaat tccgcaatgt gttattaagt tgtctaagcg
      961 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccga
//
