LOCUS       sequence-153883-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: HUNK_pCSdest
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag aagggccgtc aaggcccacc atgccggcgg cggcggggga cgggctcctg
      181 ggggagccgg cggcgcctgg gggcggcggc ggcgcggagg acgcggccag gcccgcggcg
      241 gcctgcgagg gaagtttcct gcctgcctgg gtgagcggcg tgccccgcga gcggctccgc
      301 gacttccagc accacaagcg cgtgggcaac tacctcatcg gcagcaggaa gctgggcgag
      361 ggctcctttg ccaaggtgcg cgaggggctg cacgtgctga ccggggagaa ggtggccata
      421 aaagtcattg ataagaagag agccaaaaag gacacctatg tcaccaaaaa cctgcggcga
      481 gagggtcaga tccagcagat gatccgccac cccaatatca ctcagctcct tgatatttta
      541 gaaacggaaa acagctacta cctggtcatg gagctgtgcc ctgggggcaa cctgatgcac
      601 aagatctatg agaagaagcg gctggaggag tccgaagccc gcagatacat ccgacagctc
      661 atctctgccg tagagcacct gcaccgggcc ggggtggtcc acagagactt gaagatagag
      721 aatttgctac tagatgaaga caataatatc aagctgattg actttggttt gagca
//
