LOCUS       sequence-153884-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MRAP_pCSdest
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(656..680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(748..767)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggc caacgggacc aacgcctctg ccccatacta cagctatgaa
      181 tactacctgg actatctgga cctcattccc gtggacgaga agaagctgaa agcccacaaa
      241 cattccatcg tgatcgcatt ctgggttagc ctggctgcct tcgtggtgct gctcttcctc
      301 atcttgctct acatgtcctg gtccgcctcc ccgcagatga ggaacagccc caagcaccac
      361 caaacatgcc cctggagtca cggcctcaac ctccacctct gcatccagaa gtgcctgccg
      421 tgccacaggg aacccctggc aacctcacag gctcaggcga gctcagtgga gccagggagc
      481 agaactggcc ctgaccagcc gctacgacag gagagctcct ccacgttgcc cctcgggggt
      541 ttccagaccc accccactct cctctgggaa ctgaccctca atgggggtcc cctcgtcagg
      601 agcaagccca gcgagcctcc ccctggagac aggacctctc aattgcagag ctagaaccca
      661 gctttcttgt acaaagtggt gatcctctcg agcctctaga actatagtga gtcgtattac
      721 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      781 tgaaaa
//
