LOCUS       sequence-153885-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MRAP_pCSdest
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..57
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(580..604)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(658..676)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(659..676)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 actcactata gttctagagg ctcgagagga tcaccacttt gtacaagaaa gctgggttct
       61 agctctgcaa ttgagaggtc ctgtctccag ggggaggctc gctgggcttg ctcctgacga
      121 ggggaccccc attgagggtc agttcccaga ggagagtggg gtgggtctgg aaacccccga
      181 ggggcaacgt ggaggagctc tcctgtcgta gcggctggtc agggccagtt ctgctccctg
      241 gctccactga gctcgcctga gcctgtgagg ttgccagggg ttccctgtgg cacggcaggc
      301 acttctggat gcagaggtgg aggttgaggc cgtgactcca ggggcatgtt tggtggtgct
      361 tggggctgtt cctcatctgc ggggaggcgg accaggacat gtagagcaag atgaggaaga
      421 gcagcaccac gaaggcagcc aggctaaccc agaatgcgat cacgatggaa tgtttgtggg
      481 ctttcagctt cttctcgtcc acgggaatga ggtccagata gtccaggtag tattcatagc
      541 tgtagtatgg ggcagaggcg ttggtcccgt tggccatggt gcctgctttt ttgtacaaac
      601 ttgtgatcct tgaattcgaa tcgatgggat cctgcaaaaa gaacaagtag cttgtattct
      661 atagtgtcac ctaaatcaag cttgctccga cgtccccagg cagaatggcg gttccctaaa
      721 cgagcattgc ttatatagac ctcccattag gcacgcctac cgcccattta cgtcaatgga
      781 acgcccattt gcgtcattgc ccctccccat tgacgtca
//
