LOCUS       sequence-153886-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf37_pCSdest
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(577..601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(669..688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcacctggga gcctgctgag tgttccctgt tcccacctcc aatctgctcc
      181 atcctccagc tgcatttcat tccggatcta ccagcaggat ccaactttac tactgtcatg
      241 gagggaatta cagtaattct accaactttt gaggcaacct tcttctgaca ctgtgtgaaa
      301 cagactgaaa agactctaaa gatggagaga gctcaatgct tgagaaaagg aattctgtcc
      361 tttgaaaggt ctctagaaca aaggagtctt attctgtcac ctaggctgga gtacagtggt
      421 gcaatcacag ctcactgcag cctcgatctc ctggactcaa ctaatcctcc tgcctcagcc
      481 ttctgggtag ttgagactac agaagatgag agagagaaaa agagagaaat tacagagtga
      541 cggagaaggc agacatcatg caacatgctc aagggaaccc agctttcttg tacaaagtgg
      601 tgatcctctc gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa
      661 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaa
//
