LOCUS       sequence-153889-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: FAM165B_pCSdest
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(243..267)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(321..339)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(322..339)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gttctagtta tcttttttct ctgattgctt cttcctttca gcctccagtt tttgttgttt
      121 tgcctccaac ctttttcttt gatacatttt caccccagca aatgtcaggc agagaattaa
      181 tgtttttgct gccaagatat acagcagcag gggcacgtgc tcaagaacct tccaattcat
      241 ggtgcctgct tttttgtaca aacttgtgat ccttgaattc gaatcgatgg gatcctgcaa
      301 aaagaacaag tagcttgtat tctatagtgt cacctaaatc aagcttgctc cgacgtcccc
      361 aggcagaatg gcggttccct aaacgagcat tgcttatata gacctcccat taggcacgcc
      421 taccgcccat ttacgtcaat ggaacgccca tttgcgtcat tgcccctccc cattgacgtc
      481 aatggggatg tacttggcag ccatcgcggg ccatttaccg ccattgacgt caatgggagt
      541 actgccaatg taccctggcg tacttccaat agtaatgtac ttgccaagtt actattaata
      601 gatattgatg tactgccaag tgggccattt accgtcattg acgtcaatag ggggcgtgag
      661 aacggatatg aatgggcaat gagccatccc attgacgtca atggtgggtg gtcctattga
      721 cgtcaatggg cattgagcca ggcgggccat ttaccgtaat tgacgtcaat gggggaggcg
      781 ccatatacgt caataggacc gcccatatga cgtcaatagg aa
//
