LOCUS       sequence-153891-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ERG_pCSdest
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggc cagcactatt aaggaagcct tatcagttgt gagtgaggac
      181 cagtcgttgt ttgagtgtgc ctacggaacg ccacacctgg ctaagacaga gatgaccgcg
      241 tcctcctcca gcgactatgg acagacttcc aagatgagcc cacgcgtccc tcagcaggat
      301 tggctgtctc aacccccagc cagggtcacc atcaaaatgg aatgtaaccc tagccaggtg
      361 aatggctcaa ggaactctcc tgatgaatgc agtgtggcca aaggcgggaa gatggtgggc
      421 agcccagaca ccgttgggat gaactacggc agctacatgg aggagaagca catgccaccc
      481 ccaaacatga ccacgaacga gcgcagagtt atcgtgccag cagatcctac gctatggagt
      541 acagaccatg tgcggcagtg gctggagtgg gcggtgaaag aatatggcct tccagacgtc
      601 aacatcttgt tattccagaa catcgatggg aaggaactgt gcaagatgac caaggacgac
      661 ttccagaggc tcacccccag ctacaatgcc gacatccttc tctcacatct ccactacctc
      721 agagagactc ctcttccaca tttgacttca gatgatgttg ataa
//
