LOCUS       sequence-153893-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: APP_pCSdest
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(466..477)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gctccaccat gctgcccggt ttggcactgc tcctgctggc cgcctggacg
      181 gctcgggcgc tggaggtacc cactgatggt aatgctggcc tgctggctga accccagatt
      241 gccatgttct gtggcagact gaacatgcac atgaatgtcc agaatgggaa gtgggattca
      301 gatccatcag ggaccaaaac ctgcattgat accaaggaag gcatcctgca gtattgccaa
      361 gaagtctacc ctgaactgca gatcaccaat gtggtagaag ccaaccaacc agtgaccatc
      421 cagaactggt gcaagcgggg ccgcaagcag tgcaagaccc atccccactt tgtgattccc
      481 taccgctgct tagttggtga gtttgtaagt gatgcccttc tcgttcctga caagtgcaaa
      541 ttcttacacc aggagaggat ggatgtttgc gaaactcatc ttcactggca caccgtcgcc
      601 aaagagacat gcagtgagaa gagtaccaac ttgcatgact acggcatgtt gctgccctgc
      661 ggaattgaca agttccgagg ggtagagttt gtgtgttgcc cactggctga agaaagtgac
      721 aatgtggatt ctgctgatgc ggaggaggat gactcggatg tctggtgg
//
