LOCUS       sequence-153897-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ITGB2_pCSdest
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgct gggcctgcgc cccccacttc tcgccctggt ggggctgctc
      181 tccctcgggt gcgtcctctc tcaggagtgc acgaagttca aggtcagcag ctgccgggaa
      241 tgcatcgagt cggggcccgg ctgcacctgg tgccagaagc tgaacttcac agggccgggg
      301 gatcctgact ccattcgctg cgacacccgg ccacagctgc tcatgagggg ctgtgcggct
      361 gacgacatca tggaccccac aagcctcgct gaaacccagg aagaccacaa tgggggccag
      421 aagcagctgt ccccacaaaa agtgacgctt tacctgcgac caggccaggc agcagcgttc
      481 aacgtgacct tccggcgggc caagggctac cccatcgacc tgtactatct gatggacctc
      541 tcctactcca tgcttgatga cctcaggaat gtcaagaagc taggtggcga cctgctccgg
      601 gccctcaacg agatcaccga gtccggccgc attggcttcg ggtccttcgt ggacaagacc
      661 gtgctgccgt tcgtgaacac gcaccctgat aagctgcgaa acccatgccc caacaaggag
      721 aaagagtgcc agcccccgtt tgccttcagg cacgtgctga agctgaccaa caactccaac
      781 cagtttcaga ccgaggtcgg ga
//
