LOCUS       sequence-153900-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TFF2_pCSdest
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..57
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(451..475)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(529..547)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(530..547)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 actcactata gttctagagg ctcgagagga tcaccacttt gtacaagaaa gctgggtttt
       61 agtaatggca gtcttccaca gacttcggga agaagcacca gggcacttca aagatgaagt
      121 tggagaagca gcacttccga gaggcgcatt cctcggggct gatgcccggg tagccacagt
      181 ttcttcggtc tgagacctcc atgacgcact gatccgactc ttgctttggg agggggtgga
      241 aacaccaggg gaccccagtg acactggagt cgaaacagca tccattgtca aaacactggt
      301 cactggtgat tccagggaag ccgcagttcg tcctgttatg ggggctcagc ctggagcact
      361 ggcaggggga gggtttctca ctccccgcca gggcacatag ccccaggacg aggagcgctg
      421 ccaggagctg ggcgtctcgc cgtcccatgg tgcctgcttt tttgtacaaa cttgtgatcc
      481 ttgaattcga atcgatggga tcctgcaaaa agaacaagta gcttgtattc tatagtgtca
      541 cctaaatcaa gcttgctccg acgtccccag gcagaatggc ggttccctaa acgagcattg
      601 cttatataga cctcccatta ggcacgccta ccgcccattt acgtcaatgg aacgcccatt
      661 tgcgtcattg cccctcccca ttgacgtcaa tggggatgta cttggcagcc atcgcgggcc
      721 atttaccgcc attgacgtca atgggagtac tgccaatgta ccctggcgta cttccaatag
      781 taatgtactt gccaagttac tattaataga tattgatgta ctgccaagtg ggccatttac
      841 cgtcattgac gtcaatagg
//
