LOCUS       sequence-153905-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: FTCD_pCSdest
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     134..151
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     135..151
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcttgtaaaa cgacggccag taactataac ggtcctaagg tagcgaggcc
      181 tgggtggcga attcggcacg aggcatctgc ccagagagga gcatctggat caaggtgctg
      241 gccatgtccc agctggtgga atgcgtcccc aacttttcgg aggggaagaa ccaggaggtg
      301 atcgacgcca tctctggagc catcacacag accccgggct gcgtgctgct ggatgtggac
      361 gcaggccctt ccaccaaccg caccgtgtac accttcgtgg ggccgccgga gtgcgtggtg
      421 gagggggccc tcaacgctgc ccgggtagct tcccgactta tcgacatgag caggcaccaa
      481 ggagagcacc cccgcatggg ggccctagac gtctgcccct tcatccccgt gaggggcgtc
      541 agcgtggatg agtgtgtgct ctgcgcccag gcctttggcc agaggctggc agaggagctg
      601 gacgtgccag tttacctgta cggcgaggca gccaggatgg acagtcgccg gaccctgccg
      661 gccatccggg ccggggagta cgaggccctc cctaagaagc tccagcaggc cgactgggcg
      721 cccgactttg gtcccagctc ctttgtcccc agttgggggg ccacggccac gggggcgagg
      781 a
//
