LOCUS       sequence-153909-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: Col18a1_pCSdest
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt
       61 tttgcaggat cccatcgatt cgaattcaag gatcacaagt ttgtacaaaa aagcaggctg
      121 gtaccggtcc ggaattcccg ggattcaccg aggagctggg tgcagcatcc gggcgcagac
      181 ggcctggcct ggggagatgg cgcccaggtg gcacctcctg gatgtgctca ccagtttggt
      241 cttgctgctg gtggcacgcg tctcctgggc agagccagag aatgttgctg aggaggtggg
      301 gctgctgcag ctccttggag accccctacc tgagaagatc tcacaaatcg atgaccctca
      361 cgtcgggccg gcctacatct ttggaccaga ctccaacagt ggccaggtgg cccagtatca
      421 tttcccaaaa ctcttcttcc gggacttttc gctgctgttt catgtccggc cagccacaga
      481 ggcagcaggg gtgctatttg ccatcacaga tgctgcccag gtggtagtct cactgggcgt
      541 gaagctctca gaggtccgag atggacagca aaacatctca ttgctctaca cggagcctgg
      601 ggccagccag acccagacgg gagccagctt ccgcctacct gcatttgttg ggcagtggac
      661 acacttcgcg ctcagcgtcg acggaggctc tgtggctctc tacgtagact gtgaagaatt
      721 ccaga
//
