LOCUS       sequence-153917-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: YBEY_pCSdest
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(503..527)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    571..705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(595..614)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     649..668
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatgag tttggtgatt agaaatctgc agcgagtcat ccccatcagg
      181 agagcgccac ttcgcagtaa gatcgagatt gtaaggagga ttttaggagt gcagaaattt
      241 gacctgggga tcatctgtgt tgacaacaag aatattcagc acattaatag aatctacaga
      301 gatagaaatg tcccaaccga tgtgctttct tttccatttc atgagcatct gaaagcaggt
      361 gaatttcccc agcctgattt tccagatgac tacaatttgg gagacatttt cctaggagtg
      421 gagtatatct tccatcagtg taaagaaaat gaagattaca atgacgtcct gactgtaagc
      481 ggggatgctg aaatcatata gaacccagct ttcttgtaca aagtggtgat cctctcgagc
      541 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
      601 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      661 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      721 attcatttta tgtttcaggt tcagg
//
