LOCUS       sequence-153918-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: YBEY_pCSdest
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(432..456)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(510..528)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(511..528)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gttctatatg atttcagcat ccccgcttac agtcaggacg tcattgtaat cttcattttc
      121 tttacactga tggaagatat actccactcc taggaaaatg tctcccaaat tgtagtcatc
      181 tggaaaatca ggctggggaa attcacctgc tttcagatgc tcatgaaatg gaaaagaaag
      241 cacatcggtt gggacatttc tatctctgta gattctatta atgtgctgaa tattcttgtt
      301 gtcaacacag atgatcccca ggtcaaattt ctgcactcct aaaatcctcc ttacaatctc
      361 gatcttactg cgaagtggcg ctctcctgat ggggatgact cgctgcagat ttctaatcac
      421 caaactcatg gtgcctgctt ttttgtacaa acttgtgatc cttgaattcg aatcgatggg
      481 atcctgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc
      541 gacgtcccca ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt
      601 aggcacgcct accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gcccctcccc
      661 attgacgtca atggggatgt acttggcagc catcgcgggc catttaccgc cattgacgtc
      721 aatgggagta ctgccaatgt accctggcgt acttccaata gtaatgtact tgccaagtta
      781 ctattaatag atattgatgt actgccaagt gggccattta ccgtcattga cgtcaatagg
//
