LOCUS       sequence-153919-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE167
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    77..132
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
       61 atactgtatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattact
      121 atttacaatt acaatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
      181 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
      241 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
      301 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
      361 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
      421 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
      481 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
      541 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
      601 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
      661 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
      721 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
      781 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
      841 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
      901 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
      961 gagcttggtt gacggc
//
