LOCUS       sequence-153931-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline 
            Repressor + GalL promoter driven Cas9 for yeast expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Fu BX, St Onge RP, Fire AZ, Smith JD
  TITLE     Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo.
  JOURNAL   Nucleic Acids Res. 2016 May 19. pii: gkw417.
  PUBMED    27198218
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 May 19. pii: gkw417."
COMMENT     Sequence Label: pRS416gT-GalL-Cas9
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtactccatt gggctcgata tcggcacaaa cagcgtcggt tgggccgtca ttacggacga
       61 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
      121 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
      181 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
      241 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
      301 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
      361 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
      421 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
      481 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
      541 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
      601 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
      661 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
      721 gtcactcggg ctgaccccca actttaaatc taacttcgac ctg
//
