LOCUS       sequence-153932-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline 
            Repressor + GalL promoter driven Cas9 for yeast expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Fu BX, St Onge RP, Fire AZ, Smith JD
  TITLE     Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo.
  JOURNAL   Nucleic Acids Res. 2016 May 19. pii: gkw417.
  PUBMED    27198218
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 May 19. pii: gkw417."
COMMENT     Sequence Label: pRS416gT-GalL-Cas9
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    338..356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        429..504
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      517..579
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
ORIGIN
        1 cttttgctcg ggggatctgc caattgaaca taacatggta gttacatata ctagtaatat
       61 ggttcggcac acattaaaag tataaaaact atctgaatta cgaattacat atattggtca
      121 taaaaatcaa tcaatcatcg tgtgttttat atgtctctta tctaagtata agaatatcca
      181 tagttaatat tcacttacgc taccttttaa cctgtaatca ttgtcaacag gatatgttaa
      241 cgacccacat tgataaacgc tagtatttct ttttcctctt cttattggcc ggctgtctct
      301 atactcccct atagtctgtt tcttttcgtt tcgattgtcc ctatcagtga tagagatggc
      361 gcacatggta cgctgtggtg ctcgcggctg ggaacgaaac tctgggagct gcgattggca
      421 gcggccgcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      481 gaaaaagtgg caccgagtcg gtgctttttt ctcgagccat atccaacttc caatttaatc
      541 tttctttttt aattttcact tatttgcgat acagaaagaa aaaagcgata gtaactattg
      601 aattttgttt ggatttggtt agattagata tggtttctct ttatatttac atgctaaaaa
      661 tgggctacac cagagataca taattagata tatatacgcc agtacacctt atcggcccaa
      721 gccttgtccc aaggcagcgt tttgttcttg gaaacgctgc cctacacgtt cgctatgctt
      781 caagaacttt
//
