LOCUS       sequence-153943-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCYT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Chidley C, Trauger SA, Birsoy K, O'Shea EK
  TITLE     The anticancer natural product ophiobolin A induces cytotoxicity by 
            covalent modification of phosphatidylethanolamine.
  JOURNAL   Elife. 2016 Jul 12;5. pii: e14601. doi: 10.7554/eLife.14601.
  PUBMED    27403889
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-12"; volume: "5. pii: e14601. doi"; pages: " 
            10.7554/eLife.14601"
COMMENT     Sequence Label: pLJM1-PCYT2
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..43
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
       61 gatccgctag cgctaccggt atggactaca aggatgacga tgacaagggt ggtggtggtg
      121 gtgcgatgat ccggaacggg cgcggggctg caggcggcgc agagcagccg ggcccggggg
      181 gcaggcgcgc cgtgagggtg tggtgcgatg gctgctatga catggtgcat tacggccact
      241 ccaaccagct gcgccaggca cgggccatgg gtgactacct catcgtaggc gtgcacaccg
      301 atgaggagat cgccaagcac aaggggcccc cggtgttcac tcaggaggag agatacaaga
      361 tggtgcaggc catcaaatgg gtggacgagg tggtgccagc ggctccctac gtcactacac
      421 tagagaccct ggacaaatac aactgtgact tctgtgttca cggcaatgac atcaccctga
      481 ctgtagatgg ccgggacacc tatgaggaag taaagcaggc tgggaggtac agagaatgca
      541 agcgcacgca aggggtgtcc accacagacc tcgtgggccg catgctgctg gtaaccaaag
      601 cccatcacag cagccaggag atgtcctctg agtaccggga gtatgcagac agttttggca
      661 agtgccctgg tgggcggaac ccctggaccg gggtatccca gttcctgcag acatctcaga
      721 agatcatcca gtttgcttct gggaaggagc cccagccagg ggagacagtc atctatgtgg
      781 ctggtgcctt cgacctgttc cacatcgggc atgtggactt cctggagaag gtgcacaggc
      841 tggcagagag gccctacatc atcgcgggct tacactttga ccaggaggtc aatcactaca
      901 aggggaagaa ctaccccatc atgaatctgc atgaacggac tctgagcgtg ctggcctgcc
      961 ggtacgtgtc agaagtggtg attggagccc cgtacgcggt cacagcagag ctcctaagtc
     1021 acttcaa
//
