LOCUS       sequence-153949-        2133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Light-activated Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: PA-Cdc42
FEATURES             Location/Qualifiers
     source          1..2133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    40..64
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        80..189
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             210..227
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(234..236,237..947)
                     /codon_start=1
                     /product="photostable derivative of mVenus (Lee et al., 
                     2020)"
                     /label=mGold
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(276..297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(537..556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     884..905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             966..1394
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             1998..2030
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             2037..2060
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaattggatc ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa
       61 ttccccggag ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata
      121 agaattatta tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta
      181 tttacaatca aaggagatat accatggcac accatcacca ccatcacggt tctgtgagca
      241 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      301 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      361 ccctgaagtt catcagcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      421 ccctcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      481 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      541 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      601 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      661 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
      721 caaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
      781 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
      841 accagtccaa actgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      901 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt tctggaagtg
      961 gatccttggc tactacactt gaacgtattg agaagaactt tgtcattact gacccaagat
     1021 tgccagataa tcccattata ttcgcgtccg atagtttctt gcagttgaca gaatatagcc
     1081 gtgaagaaat tttgggaaga aactgcaggt ttctacaagg tcctgaaact gatcgcgcga
     1141 cagtgagaaa aattagagat gccatagata accaaacaga ggtcactgtt cagctgatta
     1201 attatacaaa gagtggtaaa aagttctgga acctctttca cttgcagcct atgcgagatc
     1261 agaagggaga tgtccagtac tttattgggg ttcagttgga tggaactgag catgtccgag
     1321 atgctgccga gagagaggga gtcatgctga ttaagaaaac tgcagaaaat attgatgagg
     1381 cggcaaaaga acttattaag tgtgttgttg tgggcgatgg tgctgttggt aaaacatgtc
     1441 tcctgatatc ctacacaaca aacaaatttc catcggaata tgtaccgact gtttttgaca
     1501 actatgcagt cacagttatg attggtggag aaccatatac tcttggactt tgggatactg
     1561 cagggctaga ggattatgac agattacgac cgctgagtta tccacaaaca gatgtatttc
     1621 tagtctgttt ttcagtggtc tctccatctt catttcacca cgtgaaagaa aagtgggtgc
     1681 ctgagataac tcaccactgt ccaaagactc ctttcttgct tgttgggact caaattgatc
     1741 tcagagatga cccctctact attgagaaac ttgccaagaa caaacagaag cctatcactc
     1801 cagagactgc tgaaaagctg gcccgtgacc tgaaggctgt caagtatgtg gagtgttctg
     1861 cacttacaca gaaaggccta aagaatgtat ttgacgaagc aatattggct gccctggagc
     1921 ctccagaacc gaagaagagc cgcaggtgtg tgctgctatg aaagcttgcg gccgcacagc
     1981 tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat ctcgagcacc
     2041 accatcacca tcaccatcac taagtgatta acctcaggtg caggctgcct atcagaaggt
     2101 ggtggctggt gtggccaatg ccctggctca caa
//
