LOCUS       sequence-153956-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression of Ct biosensor and WspR WT under individual T7 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pETDuet-T7-Ct-WspR
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat acctatgcac aaccctcatg agagcaagac
      181 cgacctgggc gcccctctgg acggcgcggt catggtactg cttgtcgatg atcaggccat
      241 gatcggagag gctgtgcgtc gctcactggc gagcgaggcg ggcatcgact tccatttctg
      301 ttccgatccg cagcaggcgg tggcggtggc caaccagatc aagccgacgg tgatcctcca
      361 ggacctggtg atgcccggcg tcgacggcct cacgctgctc gctgcctacc gcggcaaccc
      421 ggcgacccgc gacatcccga tcatcgtcct gtcgaccaag gaagagccga cggtgaagag
      481 cgcggcgttc gccgccggcg ccaacgacta cctggtcaag ctgccggacg ccatcgagct
      541 ggtggcgcgg atccgctacc actcgcgttc gtacatcgcc ctgcagcaac gcgacgaggc
      601 ctatcgggcg ctgcgcgaga gccagcagca gttgctggag accaacctgg tgctgcagcg
      661 gctgatgaac tccgacggcc tgaccgggct ctccaaccgt cgtcacttcg acgaatacct
      721 ggagatggag tggcgccgct cgctgcgcga gcagtcgcaa ctgtcgctgc tgatgatcga
      781 cgtggactat ttcaagagct acaacgacac cttcggccac gtcgccggcg acgaggccct
      841 gcgccaggtc gccggggcca tccgcgaggg ctgcagtcgc tcctcggacc tggcggctcg
      901 gtacggcggc gaggagttcg ccatggtcct cccgggcacc tcgccgggcg gcgcacggtt
      961 gctggcggag aaggtccggc gcacggtgga gagcctgcag atcagccacg accagccacg
     1021 ccc
//
