LOCUS       sequence-153957-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses YhjH under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pCOLA-T7-YhjH
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        122..140
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    141..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             221..238
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        352..370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    371..395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
       61 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa
      121 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt
      181 ttgtttaact ttaataagga gatataccat gggcagcagc catcaccatc atcaccacag
      241 ccaggatccg aattcgagct cggcgcgcct gcaggtcgac aagcttgcgg ccgcataatg
      301 cttaagtcga acagaaagta atcgtattgt acacggccgc ataatcgaaa ttaatacgac
      361 tcactatagg ggaattgtga gcggataaca attccccatc ttagtatatt agttaagtat
      421 aagaaggaga tatacatatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa
      481 gcatcgagag cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc
      541 agccgatcta tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc
      601 atcccttgaa cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca
      661 gccatcggat ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct
      721 ttatagagca cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc
      781 gtcagcaacc aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg
      841 tggagcatat ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc
      901 tgtggctgga tgattttggt accggg
//
