LOCUS       sequence-153958-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses YhjH under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pCOLA-T7-YhjH
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(296..343)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     339..357
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(395..439)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
       61 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      121 aggggttccg cgcacatttc cccgaaaagt gccacttgcg gagacccggt cgtcagcttg
      181 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt
      241 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggt ttcagcaaaa
      301 aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcggtgg
      361 cagcagccta ggttaattaa gctgcgctag tagacgagtc catgtgctgg cgttcaaatt
      421 tcgcagcagc ggtttcttta ccagactcga gttatagcgc cagaaccgcc gtattcagcg
      481 tttctatcgg tgccgggcgt gaaagaaacc agccttgtgc ggcgaatgcg ggcgagttct
      541 gaacatcacg ccactcttcc ggcgtttcta cgccctcgac aatcaccccg cgacaatagc
      601 gattcattag atgtaaaagc tgagaaaaga gtgtgcgtcc ttccggcgac tgacgcagca
      661 tcacaaacag ttctcgcgcg attttgatgt agtcataacg cacttcactt agcgcagaga
      721 aatttgccat cccggtacca aaatcatcca gccacagcgg gccaaattca cacatcgagg
      781 caaaggttga atctttcggc agacggatat gctccaccag ttcgaaacgc agccagggaa
      841 gacgctcaat ctggcgcagg atttttggtt gctgacgcag ggcgatgagc gtagggccat
      901 caatattgac cgatgccagc aggccgtgct ctataaaga
//
