LOCUS       sequence-153959-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose induced expression of Lac repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: pBAD_Kan-LacI
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             168..188
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(214..233)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
       61 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
      121 actctctact gtttctccat acccgttttt ttgggctaga aataattttg tttaacttta
      181 agaaggagtc tagaatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
      241 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
      301 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
      361 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
      421 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
      481 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
      541 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
      601 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
      661 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
      721 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
      781 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
      841 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
      901 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
      961 ccattaccga gtccgggctg c
//
