LOCUS       sequence-153960-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose induced expression of Lac repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: pBAD_Kan-LacI
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..128
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(184..201)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
       61 cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct
      121 gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagctggaga ccgtttaaac
      181 tcaatggtga tggtgatgat gaccgggctg cagctgcccg ctttccagtc gggaaacctg
      241 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      301 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
      361 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
      421 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
      481 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
      541 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
      601 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
      661 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
      721 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
      781 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
      841 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
      901 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
      961 caccgccg
//
