LOCUS       sequence-153961-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4->QF2 HACK construct compatible with GMR attP integrated 
            insertions. Contains 5' and 3' GAL4 homology arms and gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pBPGUw-HACK-G4>QF2
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     83..101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    171..204
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             244..681
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
       61 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagtacg
      121 aagtttctat actttctaga gaatagaaac ttctacgtaa cgcgagatct gaagttccta
      181 ttctctagaa agtataggaa cttctaggga taacagggta atagatctgg atcctaatga
      241 tgaaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
      301 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
      361 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
      421 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt
      481 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
      541 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
      601 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
      661 caaagacagt tgactgtatc gattgactcg gcagctcatc atgataactc cacaattccg
      721 ttggatttta tgcccaggga tgctcttcat ggatttgatt ggtctgaaga ggatgacatg
      781 tcggatggct tgcccttcct gaaaacggac cccaacaata atgggttctt tggcgacggt
      841 tctctcttat gtattcttcg atctattggc tttaaaccgg aaaattacac gaactctaac
      901 gttaacaggc tcccgaccat gattacggat agatacacgt tggcttctag
//
