LOCUS       sequence-153964-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual selection plasmid with lac operator, selection for binding with
            spectinomycin, for non-binding with chloramphenicol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: pSAH0016
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      302..333
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
       61 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
      121 ccaccgctgc gttcggtcaa ggttctggac cagttgcgta agcgcatttt agcttcctta
      181 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
      241 tgaaagttgg aacctcttac ctgcaggagt cactaagggt tagttagtta gattagcaga
      301 aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt tatcattggg
      361 gctcactcaa aggcggtaat cagataaaaa aaatccttag ctttcgctaa ggatgatttc
      421 tgctagtgtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac caaaaactcg
      481 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt
      541 tccctttgat atgtaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc
      601 actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct
      661 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatacttac
      721 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
      781 catcgtgtag tgtttttctt agtccgttat gtaggtagga atctgatgta atggttgttg
      841 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
      901 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
      961 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tgg
//
