LOCUS       sequence-153965-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4->QF2 HACK construct compatible with GMR attP integrated 
            insertions. Contains 5' and 3' GAL4 homology arms and gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pBPGUw-HACK-G4>QF2
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        182..257
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        258..678
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        699..774
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggattcctat ccggcggtag tcccatcatt ggcatggtag gtacccgaaa gcaacccctt
       61 agttacagac acagcgcgta cgtccttcgc atccttatga ttcccaagta catattctgc
      121 aagagtacag tatatatagg aaagatatcc gggtgaactt cgatgtgcag cgtaccacaa
      181 cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      241 tggcaccgag tcggtgcttt tttgctcacc tgtgattgct cctactcaaa tacaaaaaca
      301 tcaaattttc tgtcaataaa gcatatttat ttatatttat tttacaggaa agaattcctt
      361 ttaaagtgta ttttaaccta taatgaaaaa cgattaaaaa aaatacataa aataattcga
      421 aaatttttga atagcccagg ttgataaaaa ttcatttcat acgttttata acttatgccc
      481 ctaagtattt tttgaccata gtgtttcaat tctacattaa ttttacagag tagaatgaaa
      541 cgccacctac tcagccaaga ggcgaaaagg ttagctcgcc aagcagagag ggcgccagtg
      601 ctcactactt tttataattc tcaacttctt tttccagact cagttcgtat atatagacct
      661 attttcaatt taacgtcgtg tattctgaga aagctggagt tttagagcta gaaatagcaa
      721 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      781 gcctacctgg agcctgagag ttgttcaata aaataaaaat gtttcgtttt tttgctttcg
      841 ccagtattta ttatttttca tcaatatgta ttcaatttgg tatgtattta gtaattgtaa
      901 tatatagac
//
