LOCUS       sequence-153971-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QF2 HACK backbone.  Contains QF2-hsp70, but no gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-QF2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     69..88
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    295..328
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    342..375
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             539..592
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aataggggtc agtgttacaa ccaattaacc aattctgaac attatcgcga gcccatttat
       61 acctgaatat ggctcataac accccttgtt tgcctggcgg cagtagcgcg gtggtcccac
      121 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggactc
      181 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
      241 tgggcctttc gcccgggcta attatggggt gtcgccctta ttcgactcta tagtgaagtt
      301 cctattctct agaaagtata ggaacttctg aagtggggcg ggtgccaggg cgtgcccttg
      361 ggctccccgg gcgcgtacaa gccgaaggcg cgcatgatga aataacataa ggtggtcccg
      421 tcgaaagccg aagcttaccg aagtatacac ttaaattcag tgcacgtttg cttgttgaga
      481 ggaaaggttg tgtgcggacg aatttttttt tgaaaacatt aacccttacg taacgcgtga
      541 gggccgcggc agcctgctga cctgcggcga tgtggaggag aaccccgggc cccctaggat
      601 gccacccaag cgcaaaacgc ttaacgctgc ggctgaggct aacgctcatg ccgacggaca
      661 cgccgacgga aacgccgacg gacacgtggc caatacggcc gcgtcctcga ataatgcgag
      721 gttcgctgat ctcactaaca tcgatactcc gggtctggga cccacaacta cgaccctgct
      781 cgtggaacca gcacgctcaa agcgtcaacg agtgtcccgc gcatgcgacc agtgccgtgc
      841 agcccgagag aaatgcgacg gaatacagcc tgcgtgtttc ccgtgcgttt
//
