LOCUS       sequence-153972-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 Cerulean-Zhang2.0
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(17..38)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(278..297)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             437..688
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     625..646
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(757..777)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
       61 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      121 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      181 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      241 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      301 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      361 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
      421 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      481 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      541 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      601 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      661 cactctcggc atggacgagc tgtacaagta aagcgtctgg aacaatcaac ctctggatta
      721 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
      781 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
      841 ctccttgtat aa
//
