LOCUS       sequence-153973-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and 
            gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-GAL4>QF2
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     69..88
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    295..328
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    342..375
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    544..577
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aataggggtc agtgttacaa ccaattaacc aattctgaac attatcgcga gcccatttat
       61 acctgaatat ggctcataac accccttgtt tgcctggcgg cagtagcgcg gtggtcccac
      121 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggactc
      181 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
      241 tgggcctttc gcccgggcta attatggggt gtcgccctta ttcgactcta tagtgaagtt
      301 cctattctct agaaagtata ggaacttctg aagtggggcg ggtgccaggg cgtgcccttg
      361 ggctccccgg gcgcgtacaa gccgaaggcg cgcatgatga aataacataa ggtggtcccg
      421 tcgaaagccg aagcttaccg aagtatacac ttaaattcag tgcacgtttg cttgttgaga
      481 ggaaaggttg tgtgcggacg aatttttttt tgaaaacatt aacccttacg taacgcgaga
      541 tctgaagttc ctattctcta gaaagtatag gaacttctag ggataacagg gtaatagatc
      601 tggatcctaa tgatgaaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      661 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      721 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      781 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      841 ccttgacatg attttgaa
//
