LOCUS       sequence-153974-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits VPR to compatible gRNAs for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_VPR
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..129)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(166..735)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattgtc gacttaacgc gttgattgtc
       61 gacttaaccg gtactagttc attactacgt agaatcgaga ccgaggagag ggttagggat
      121 aggcttaccg atatcaacca ctttgtacaa gaaagctggg tttcaaaaca gagatgtgtc
      181 gaagatggac agtcctgtgc tgatatgcat ggcatgcaag aggcactcgt cgttcaggaa
      241 ggtatccaga atctcgttca attccggggt caggggtgag tccaggttca gatcctcggt
      301 catggactca agtgtggttg tcagctcatc cagatggccc cttgggggcg gatgggaaag
      361 gtccatttgg ccacagattg cagcctcttc cttctgggga atcacagtat cggccatctc
      421 ccgaagggct ttgacagcct ggctcgtctc ttcatcggga tcctccaaca ggtgactggc
      481 ctcgggagtc actgcgggcg ctggatccag tggctgaggg actggtgccg gggtcagtga
      541 cccgactggc tcatgtactg gaccggttgg tgttggtgcg aggctggcgg ggagtgggcg
      601 gttggcccat ggacttcctg gaggatgaaa tggccggatt cgttttggct ggcacacctc
      661 gcggccctca aacacgtcac taatagcgga gccggcctca ggcttcggca aaaacatccc
      721 ttccctggaa tcccggctgc cgctgccaga gcccagcaag gctgagaaat ccatatcggc
      781 gatagagctg aagtcctcgt cgc
//
