LOCUS       sequence-153975-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional interference assessment; strong interfering 
            promoter, landing site for spacers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: SAH0141-0.0-conP01
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(11..17,18..33,34..39))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      94..123
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      279..373
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 agattccaca cattatacga gccgatgatt aattgtcaag agaattcgag tcactaaggg
       61 ctaactaact aattacgtag caatcaactc actggctcac cttcacgggt gggcctttct
      121 tcggcacggg caaattgctg aatattcctt ttcttagacg tcaggtggca aacgcgaagt
      181 aatcttttcg gttttaaaga aaaagggcag ggtggtgaca ccttgcccgt ttttttgccg
      241 gagctagagc tgtcagacca agtttacgag ctcgcttgga ctcctgttga tagatccagt
      301 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
      361 tattggtgag aatccaagca ctagggacag taagacgggt aagcctgttg atgataccgc
      421 tgccttactg ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc
      481 agactggaaa atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata
      541 gcagacccgc cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta
      601 gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca
      661 gagccgtgag cgcagc
//
