LOCUS       sequence-153979-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QF2 HACK backbone compatible with GMR attP integrated insertions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pBPGUw-HACK-QF2
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     83..101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             158..211
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
       61 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgta cgaagtttct
      121 atactttcta gagaatagaa acttctacgt aacgcgtgag ggccgcggca gcctgctgac
      181 ctgcggcgat gtggaggaga accccgggcc ccctaggatg ccacccaagc gcaaaacgct
      241 taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa acgccgacgg
      301 acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc tcactaacat
      361 cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag cacgctcaaa
      421 gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga aatgcgacgg
      481 aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt atcaggcttc
      541 gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg ccctcgcctg
      601 gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg tccgtgacgc
      661 cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc gactccatgc
      721 ccgttgggct actagccgtg tcaataagag cattacccgc ctcctccgtc agttggagct
      781 ccctcctacc gccacggcta cggcctcgat aatgccgcac gtgatggagc agcctctcag
      841 taccagcatt aaccccgtca acgaccgctt caacggtatt cccaacccca ctccgtataa
      901 ctccgatgca gctctcgatg ctatcactca gaccaacgat tatggaagcg ta
//
